Antioxidative defense organization in retroperitoneal white adipose tissue during acclimation to cold-The involvement of L-arginine/NO pathway
Buzadžić, Biljana J.
Korac, Aleksandra B
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1. Retroperitoneal white adipose tissue (RpWAT) antioxidative defense was investigated in untreated, L-arginine-treated and N(omega)-nitro-L-arginine methyl ester (L-NAME)-treated rats kept at 4 +/- 1 degrees C (1, 3, 7, 12, 21 and 45 days) and compared to control rats at 22 +/- 1 degrees C. 2. Cold-acclimation-induced RpWAT weight decrease was accompanied by a decline in glutathione level and increased activity of manganese superoxide dismutaise (MnSOD), glutathione S-transferase (GST), catalase, glutathione peroxidase and glutathione reductase at different time-points. 3. L-arginine accelerated RpWAT weight decrease, the increase in MnSOD and GST activities and the prolonged increase of catalase, MnSOD and GST activities. L-NAME delayed cold-induced catalase activity increase and tissue weight decrease. Prolonged L-NAME-treatment had a similar effect on RpWAT as L-arginine. 4. Results suggest the involvement Of L-arginine/NO pathway in RpWAT oxidative metabolic augmentation induced by cold-acclimation. (C) 2009 Elsevier Ltd. All rights reserved.