Efekti SIN-1 na antioksidacioni zaštitni sistem u crvenim krvnim ćelijama pacova
Effect of SIN-1 on antioxidative defence system in red blood cells of rats
Maletić, Snežana D.
Ognjanović, Branka I.
Štajn, Andraš Š.
Žikić, Radoslav V.
Article (Published version)
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The main objective of this research is to establish the effect of 3-morpholino-sydnonimine-hydrochloride (SIN-1) on antioxidative defense system (AOS) in rat red blood cells. Rats erythrocyte and reticulocyte-rich suspensions were aerobically incubated without (control) or in the presence of SIN-1 (0.1, 0.25, 0.5, 1.0 and 1.5 mM). The concentrations of non-enzymatic components and activities of enzymatic component of AOS were determined after incubation. In rat erythrocytes SIN-1 did not alter vitamin C (Vit C), while induced increase of vitamin E (Vit E) concentrations. Unaltered concentration of reduced glutathione (GSH) following by decrease of oxidized glutathione (GSSG) resulted in decrease of reduction potential of this redox couple (decrease of GSSG/2GSH ratio). These data indicate that GSH redox couple is included in defense of rat erythrocytes from oxidative damage. Activities of all AOS enzyme did not altered in the presence of SIN-1 in rat erythrocytes. In reticulocytes a high doses of SIN-1 induced a decrease of Vit C and increase of Vit E concentrations, indicating defense role of antioxidative vitamins. Level of GSH redox couple is not changed. SIN-1 inhibited activity of manganese containing superoxide dismutase (Mn SOD) which was followed by stimulation of copper zinc containing superoxide dismutase (CuZn SOD) activity. SIN-1 did not alter activities of other AOS enzyme in rat reticulocytes.