Agrobacterium-mediated transformation of two Serbian potato cultivars (Solanum tuberosum L. cv. Dragacevka and cv. Jelica)
Аутори:Cingel, Aleksandar D.
Vinterhalter, Branka S.
Vinterhalter, Dragan V
Ćalić-Dragosavac, Dušica D.
Ninković, Slavica B.
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An efficient protocol for Agrobacterium-mediated transformation of Serbian potato cultivars Dragacevka and Jelica, enabling the introduction of oryzacystatin genes OCI and OCII, was established. Starting with leaf explants, a two-stage transformation protocol combining procedures of Webb and Wenzler provided high shoot regeneration efficiency: 84 - 89% for Dragacevka cultivar and 60 - 68% for Jelica cultivar as compared to 76 - 86% for Desiree, the most frequently used cultivar in transformation experiments. PCR analysis of a small sample of putative transformants showed a nptII integration frequency of 90.9, 76.9 and 86.4% for Dragacevka, Jelica and Desiree, respectively. Regeneration and transformation efficiency was strongly genotype-dependent.