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dc.creatorMisirkić Marjanović, Maja
dc.creatorTodorović-Marković, Biljana M
dc.creatorVučićević, Ljubica
dc.creatorJanjetović, Kristina
dc.creatorJokanović, Vukoman R
dc.creatorDramicanin, Miroslav D
dc.creatorMarković, Zoran M
dc.creatorTrajković, Vladimir S
dc.description.abstractThe influence of fullerene (C-60) nanoparticles on the cytotoxicity of a highly reactive free radical nitric oxide (NO) was investigated. Fullerene nanoparticles were prepared by mechanochemically assisted complexation with anionic surfactant sodium dodecyl sulfate, macrocyclic oligosaccharide gamma-cyclodextrin or the copolymer ethylene vinyl acetate-ethylene vinyl versatate. C-60 nanoparticles were characterized by UV-vis and atomic force microscopy. While readily internalized by mouse L929 fibroblasts, C-60 nanoparticles were not cytotoxic. Moreover, they partially protected L929 cells from the cytotoxic effect of NO-releasing compounds sodium nitroprusside (SNP), S-nitroso-N-acetylpenicillamine (SNAP), S-nitrosoglutathione (GSNO) and 3-morpholino-sydnonimine (SIN-1). C-60 nanoparticles reduced SNP-induced apoptotic cell death by preventing mitochondrial depolarization, caspase activation, cell membrane phosphatidylserine exposure and DNA fragmentation. The protective action of C-60 nanoparticles was not exerted via direct interaction with NO, but through neutralization of mitochondria-produced superoxide radical in NO-treated cells, as demonstrated by using different redox-sensitive reporter fluorochromes. These data suggest that C-60 complexes with appropriate host molecules might be plausible candidates for preventing NO-mediated cell injury in inflammatory/autoimmune disorders. (C) 2009 Elsevier Ltd. All rights reserved.en
dc.description.sponsorshipMinistry of Science of the Republic of Serbia [145073]sr
dc.titleThe protection of cells from nitric oxide-mediated apoptotic death by mechanochemically synthesized fullerene (C-60) nanoparticlesen
dcterms.abstractМарковић, Зоран М; Трајковић, Владимир С; Тодоровић-Марковић, Биљана М; Вуцицевић, Љубица М; Јањетовић, Кристина Д.; Мисиркић, Маја С; Јокановић, Вукоман Р; Драмицанин, Мирослав Д;

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