Razviće mahovine Pogonatum urnigerum (Hedw.) P. Beauv. gajene u uslovima kulture in vitro
Development of the moss Pogonatum urnigerum (Hedw.) P. Beauv. under in vitro culture conditions
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Pogonatum urnigerum (Polytrichaceae) in vitro culture was established from spores collected in nature. Both protonema and gametophore stages of gametophyte development were obtained. Also, a stable callus culture was established using hormone-free nutrient medium. The best nutrient medium for development was half-strength Murashige-Skoog medium supplemented with 1.5% sucrose. Auxin treatment enabled some gametophores to develop, but prolonged treatment induced early senescence. Tissues grown on cytokinin did not produce any gametophytes and did not survive prolonged treatment.