Detection of proteins possibly involved in self-incompatibility response in distylous buckwheat
Authors:Miljuš-Đukić, Jovanka D.
Ninković, Slavica B.
Radović, Svetlana R
Maksimović, Vesna R
Brkljačić, Jelena M.
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Buckwheat ( Fagopyrum esculentum Moench) is a heterostylous plant displaying heteromorphic sporophytic self-incompatibility (SI). In order to detect proteins involved in SI, pistils from both long and short styles were isolated and then selfed or cross-pollinated. One-dimensional gel electrophoresis revealed that short pistils 2 h after selfing contained an unique 50 kDa protein. In the two-dimensional electrophoresis two distinct groups of proteins possibly involved in SI response were detected in the short, and one in the long pistils.