Antioxidative defense in the rat skin after the adaptation to cold
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(1) Activity of superoxide dismutase (CuZn- and Mn-SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), glutathione S-transferase (GST) and thioredoxin reductase (TR), and the amount of glutathione (GSH) were assayed in the skin of control (22 +/- 1degreesC), cold-adapted rats (45 days, 5 +/- 1degreesC), and cold-adapted rats brought back to room temperature after 1, 3, 7 and 15 days. (2) There were no changes in the observed components of the antioxidative defense in the skin of the cold-adapted rats. (3) During the re-adaptation of cold-adapted rats (18.8 +/- 3.1 nM GSH g(-1) tissue), only changes in the skin GSH level were found. After 1, 3 and 7 days of re-adaptation (39.5+/-5.3, 51.5+/-4.93 and 51.2+/-8.35 nM GSH g(-1) tissue, respectively) the amount of GSH was considerably increased, while after 15 days at room temperature (19.1+/-2.3 nM GSH g(-1) tissue) it declined to control levels (20.7+/-3.1 nM GSH g(-1) tissue). (C) 2003 Elsevier Science Ltd. All rights reserved.