Activity of antioxidant defense enzymes and glutathione content in some tissues of the Belgrade (b/b) laboratory rat
Korać, Bato M.
Blagojević, Duško P.
Buzadžić, Biljana J.
Petrović, Vojislav M.
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The activity of antioxidant defense (AD) enzymes-superoxide dismutase (SOD, EC 18.104.22.168.), catalase (CAT, EC 22.214.171.124.), glutathione peroxidase (GSH-Px, EC 126.96.36.199.), glutathione-S-transferase (GST, EC 188.8.131.52), glutathione reductase (GR, EC 184.108.40.206) and glutathione (GSH) content of the anemic Belgrade (b/b) laboratory rats-were measured and analyzed in liver, spleen, lung, heart, brain and testes in comparison with nonanemic controls. The activities of hepatic Mn SOD, CAT, GSH-Px and GST (P < 0.02, P < 0.01 and P < 0.005) were decreased in anemic, comparing with nonanemic animals, whereas the spleen CuZn SOD, Mn SOD, CAT and GSH-Px (P < 0.005, P < 0.02, P < 0.005 and P < 0.01) activities were increased. In the lung of anemic rats, Mn SOD, GSH-Px and GR (P < 0.005, P < 0.01, P < 0.05) activities were higher, whereas GST (P < 0.01) activity was lower in relation to nonanemic ones. In anemic rats, heart Mn SOD (P < 0.05) activity was increased, brain GSH-Px (P < 0.005) activity was lower, whereas GR (P < 0.02) activity was higher compared with nonanemic controls. CuZn SOD (P < 0.05) activity in the testes was elevated and GSH-Px (P < 0.05) reduced in anemic animals. GSH content was decreased in the liver (P < 0.01), lung and brain (P < 0.005) and increased in the spleen (P < 0.02) of anemic rats in relation to the controls. Our data suggest phenotype specific differences in the AD system of the Belgrade (b/b) rat tissues in comparison with nonanemic controls. (C) 1997 Elsevier Science Inc.