Response of alpha-glucosidase in gypsy moth larvae to acute and chronic dietary cadmium
Аутори:Vlahović, Milena S.
Mataruga, Vesna D. Peric
Lazarević, Jelica M.
Matic, Dragana R.
Todorovic, Dajana D.
Ilijin, Larisa A.
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We investigated the effects of acute and chronic treatments with cadmium at 10 mu g Cd/g dry food and 30 mu g Cd/g dry food on alpha-glucosidase activity of the 4th instar larvae of Lymantria dispar (Lepidoptera: Lymantriidae) as well as subsequent recovery. Enzyme inhibition was recorded during acute exposure to 30 mu g Cd/g dry food and during chronic treatment at the lower metal concentration. After three days recovery from 10 mu g Cd/g dry food, the alpha-glucosidase activity returned to the control level. One-way ANOVA showed that cadmium significantly influenced the activity of alpha-glucosidase during all treatments. The index of phenotypic plasticity was higher during chronic treatment at 10 mu g Cd/g dry food than at 30 mu g Cd/g as well as during the recovery. We detected four glucosidase isoforms by NATIVE PAGE. The activities and expressions of the isoforms depended on both larval genotype and cadmium treatment.
Кључне речи:enzyme isoforms; gypsy moth; Cadmium; phenotypic plasticity; digestive enzyme
Извор:Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Wastes, 2015, 50, 4, 285-292