Characterisation and preliminary lipid-lowering evaluation of Lactobacillus isolated from a traditional Serbian dairy product
Jovanovic, J. Novakovic
Petkovic, B. Janac
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We investigated the potential probiotic properties of indigenous lactic acid bacteria (LAB) isolated from Serbian homemade cheese. Seventeen LAB strains were isolated and characterised using standard protocols. One of the strains showed several probiotic properties: survival at low pH and in bile salts solution, antimicrobial activity, susceptibility to antibiotics and adhesion to hexodecane. DNA analysis identified the isolate as Lactobacillus casei, hereafter named Lactobacillus casei 5s. The lipid lowering effect of Lactobacillus casei 5s was evaluated in vivo using a hyperlipidemic rat model. Orally administered Lactobacillus casei 5s significantly decreased the elevated total serum cholesterol and triglycerides, and attenuated macro vesicular steatosis in the liver. Moreover, Lactobacillus casei 5s improved the intestinal microbial balance in favour of lactobacilli, while decreasing the number of Escherichia coli cells. The bacteria were re-isolated and identified from the surface of the intestinal mucosa and from the faecal samples of treated animals, indicating adhesiveness and colonisation ability. The results of an acute oral toxicity study in mice and the absence of translocation to other organs demonstrated the safety of the strain. In conclusion, Lactobacillus casei 5s demonstrated promising probiotic potential and might be a good candidate for more detailed investigations.
Кључне речи:probiotic; Lactobacillus casei; lipid-lowering effect; safety
Извор:Beneficial Microbes, 2015, 6, 1, 119-128