Production of guaianolides in Agrobacterium rhizogenes - transformed chicory regenerants flowering in vitro
Аутори:Bogdanovic, Milica D.
Todorovic, Sladana I.
Dragicevic, Milan B.
Verstappen, Francel W. A.
Bouwmeester, Harro J.
Simonovic, Ana D.
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Chicory (Cichorium intybus L) is rich in bitter sesquiterpene lactones, mainly guaianolides: lactucin, 8-deoxylactucin, lactupicrin and their 11(S),13-dihydroderivatives-compounds recognized for their antimicrobial and anti-cancer effects. In vitro plant tissue culture, and particularly Agro bacterium rhizogeries-generated hairy root (HR) cultures, have many advantages as systems for production of valuable secondary metabolites. Although chicory HRs grow better than control culture, having nearly 60 times greater fresh weight gain, they do not contain a higher content of guaianolides than wild type (wt) roots. Thus we have established in vitro system comprised of wt root and HR cultures, and wt and transformed regenerated plants of the same age, in rosette and flowering stage, in order to study the effects of transformation, organogenesis and flowering on guaianolides production. Both regeneration and flowering in vitro were spontaneous, so the results were not influenced by exogenous growth regulators. Some of the transformed clones grew better, but all flowered earlier in comparison to wt plants. Floral transition increased guaianolides content in both roots and leaves of transformed, but not of wt plants. Expression of RolC oncogene correlated with floral transition and with guaianolides accumulation. We propose A. rhizogenes transformed plants at the flowering stage as an alternative source of free guaianolides, where, in contrast to HRs, entire plants can be used for the extraction. (C) 2014 Elsevier B.V. All rights reserved.
Кључне речи:Sesquiterpene Lactones; Guaianolides; Hairy root; Cichorium intybus; Rol genes; Flowering
Извор:Industrial Crops and Products, 2014, 60, 52-59