COMPARATIVE PHYLOGENETIC ANALYSIS OF DOBRAVA-BELGRADE VIRUS L AND S GENETIC SEGMENTS ISOLATED FROM AN ANIMAL RESERVOIR IN SERBIA
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The Dobrava-Belgrade virus (DOBV) is a member of the Bunyaviridae family, genus Hantavirus, possessing a single-stranded RNA genome consisting of three segments, designated L (large), M (medium) and S (small). In this study, we present phylogenetic analysis of a newly detected DOBV strain isolated from Apodemus agrarius. Analysis was based on partial L and S segment sequences, in comparison to previously published DOBV sequences from Serbia and elsewhere. A phylogenetic tree based on partial S segment revealed local geographical clustering of DOBV sequences from Serbia, unrelated to host (rodent or human). The topology of the phylogenetic tree was confirmed with a high percent of completely or partially resolved quartets in likelihood-mapping analysis, whereas no evidence of possible recombination in the examined S segment data set was found.
Кључне речи:phylogenetic analysis; likelihood mapping; recombination
Извор:Archives of Biological Sciences, 2014, 66, 2, 497-506