Uticaj elektromagnetnog polja (2 mT, 50 Hz) na spontanu bioelektričnu aktivnost neurona antenalnog lobusa adulta Morimus funereus (Insecta, Coleoptera)
The influence of electromagnetic field (2 mT, 50 Hz) on spontaneous bioelectrical activity of antennal lobe neurons of adult Morimus funereus (Insecta, Coleoptera)
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External magnetic fields (MF) disturb the equilibrium of biological systems, even during short-term exposure, resulting in disturbances at different levels of organization. These changes accumulate in organisms, which is one of the reasons for testing biological effects of MF. On the other hand, the fact that modern man is exposed to increasing number of MF sources due to the use of new technologies in everyday life, industry, medicine, as well as therapeutic application of MF, makes this topic even more important, in terms of human health protection. It is assumed that the interaction between external MF and organisms takes place at all levels of organization, including the nervous system. Thus, the aim of this study was to investigate the influence of electromagnetic field (EMF) of different characteristics (exposure duration, waveform) on spontaneous bioelectrical activity of antennal lobe neurons of adult Morimus funereus. Neurons activity (background/neuronal population and those nearest to the recording electrode) was registered through several phases of exposure of adult longhorn beetles to sine wave or square wave MF of different exposure duration: 5, 10 and 15 minutes (MP-5, MP-10 and MP-15, respectively). Estimation of MF influence was based on registered changes of neuronal activity. (I)reversibility of such changes was tested through comparison the triplets formed by particular experimental phases. Changes in triplets that follow the time course of the experiment, with reference to MF influence of different exposure duration (MP-5, MP-10 and MP-15, respectively), were also tested. Results of this study showed that the applied MF, regardless of exposure duration and waveform, modifies the activity of antennal lobe neurons. In sine wave MF, the most prominent changes are recorded after MP-15, while in square wave MF such changes were recorded after MP-5. Control activity of the neuronal population was significantly changed both after MP-5 and MP-10, as well as significantly decreased after MP-15. Sine wave MF, regardless of exposure duration, significantly inhibits control activity of nearest neurons. Changes in control neuronal activity are significantly lower in square wave MF of different exposure duration (MP-5, MP-10, MP-15). Compared to the activity detected immediately after exposure to sine wave MF, the activity of neuronal population and nearest neurons is significantly increased 35 minutes after cessation of MF-5 and MP- 10 exposure, respectively. In case of square wave MF, changes in the activity of neuronal population and nearest neurons are significant 35 minutes after cessation of MF-5 exposure compared to the activity detected immediately after exposure to this MF. On the other hand, the neuronal activity detected 35 minutes after cessation of MF-10 exposure was significantly higher in the neuronal population and significantly lower in the nearest neurons compared to the activity detected immediately after exposure to this square wave MF. Changes in the activity of neurons exposed to sine wave or square wave MF of different duration were mostly irreversible. Sine wave MF of different duration does not change reversibility factors between the triplets formed by particular experimental phases. In contrast, in square wave MF, reversibility factors of the nearest neurons are significantly changed between the triplets formed by MP-10 and MP-15 experimental phase. Results of our experiment imply that M. funereus individuals are sensitive to both sine wave and square wave EMF (2 mT, 50 Hz) of different duration, whereby their reactions depend on the characteristics of the applied MF and specificity of each individual. In addition, it can be presumed that adult M. funereus has the ability to detect and respond to external MF.
Keywords:Morimus funereus; Electromagnetic field; Spontaneus bioelectrical activity; Extracellular registration; Excitation/inhibition; Irreversibility/reversibility
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Belgrade: University of Belgrade, Faculty of Biology (2013): 1-160[ Google Scholar ]