Evaluation of functionalized mesoporous silica SBA-15 as a carrier system for Ph 3 Sn(CH 2) 3 OH against the A2780 ovarian carcinoma cell line
Kaluđerović, Goran N.
© 2016 The Royal Society of Chemistry
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SBA-15|Sn3, a mesoporous silica-based material (derivative of SBA-15) loaded with an organotin compound Ph3Sn(CH2)3OH (Sn3), possesses improved antitumor potential against the A2780 high-grade serous ovarian carcinoma cell line in comparison to Sn3. It is demonstrated that both the compound and the nanostructured material are internalized by the A2780 cells. A similar mode of action of Sn3 and SBA-15|Sn3 against the A2780 cell line was found. Explicitly, induction of apoptosis, caspase 2, 3, 8 and 9 activation, accumulation of cells in the hypodiploid phase as well as accumulation of ROS were observed. Interestingly, Sn3 loaded in the mesoporous silica-based material needed to reach a concentration 3.5 times lower than the IC50 value of the Sn3 compound, pointing out a higher effect of the SBA-15|Sn3 than Sn3 alone. Clonogenic potential, growth in 3D culture as well as mobility of cells were disturbed in the presence of SBA-15|Sn3. Such behavior could be associated with the suppression of p-38 MAPK. Less profound effect of Sn3 compared to SBA-15|Sn3 could be attributed to a different regulation of p-38 and STAT-3, which are mainly responsible for an appropriate cellular response to diverse stimuli or metastatic properties.
Keywords:Cell culture; Cell death; Cells; Cytology; Organometallics; Silica; Tin; Carrier systems; Cellular response; Clonogenic potential; Functionalized; Mesoporous Silica; Mesoporous silica-based materials; Organotin compound; Ovarian carcinoma cell
Source:Dalton Transactions, 2016, 45, 47, 18984-18993
- Molecular mechanisms of physiological and pharmacological control of inflammation and cancer (RS-173013)
- Ministerio de Economía y Competitividad, Spain (Grant No. CTQ2015-66164-R
- Universidad Rey Juan Carlos-Banco de Santander (Excellence Group QUINANOAP)