Fiziološki i molekularno-genetički pokazatelji međuvrsne hibridizacije u okviru roda Centaurium Hill (Gentianaceae)
Physiological and molecular genetic indicators of interspecific hybridization within the genus Centaurium hill (Gentianaceae)
Doctoral thesis (Published version)
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Species of genus Centaurium Hill readily hybridize in natural habitats. Plenty of evidence reveal the importance of polyploidization and hybridization as evolutionary mechanisms responsible for speciation within this genus. Allotetraploids obtained from Centaurium erythraea Rafn × C. littorale (Turner) Gilmour cross under in vitro conditions supported hypothesis that pre-reproductive barriers existing in these species are poorly efficient in preventing hybridization. Implemented methodology for the detection of hybrid individuals were successfully applied in natural populations from Subotica-Horgoš sands. New taxon, named Centaurium pannonicum, is a hexaploid hybrid arisen from C. erythraea x C. littorale ssp. compressum (Hayne) Kischner cross, after fertilization, i.e. joining of diploid and tetraploid nuclei. De novo developed EST-SSR markers suggested that hybrid taxon converges to C. erythraea. As revealed by the selected set of phytochemical markers, C. pannonicum was more similar to C. littorale ssp. compressum. Developed sets of EST-SSR and phytochemical markers are transferable to other Centaurium species and could facilitate further investigation of interspecific hybridization within the genus. C. pannonicum maintains its hexaploidy in natural populations by self-fertilization and/or other forms of positive assortative crossing.
Keywords:Centaurium; Chemotaxonomic markers; EST-SSR; Interspecific hybridization; Polymorphism; Polyploidy; RAPD
Source:University of Belgrade, Faculty of Biology, 2016, 1-200
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