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dc.creatorVinterhalter, Branka
dc.creatorMitić, Nevena
dc.creatorVinterhalter, Dragan
dc.creatorUzelac, Branka
dc.creatorKrstić-Milošević, Dijana
dc.date.accessioned2018-02-14T10:12:53Z
dc.date.available2018-02-14T10:12:53Z
dc.date.issued2016
dc.identifier.urihttps://www.degruyter.com/view/j/biolog.2016.71.issue-2/biolog-2016-0020/biolog-2016-0020.xml
dc.identifier.urihttp://ibiss-r.rcub.bg.ac.rs/123456789/2985
dc.description.abstractStudy describes protocols for in vitro propagation of Gentiana utriculosa L. via axillary shoot multiplication and indirect somatic embryogenesis. Shoot cultures were established from seedling epicotyl explants cultured on MS medium supplemented with 0.25 mg L-1 BA and 0.1 mg L-1 IAA. Medium containing 2% sucrose and 0.2 mg L-1 BA improved multiple shoot production, providing 2.3 shoots per explant. The highest rooting (29.6%) was obtained on medium with 1/2 MS mineral salts and 0.5 mg L-1 NAA. Somatic embryogenesis was induced using different explants, including immature seeds as well as leaves and roots from shoot cultures. Following auxin treatment with either 1.0 mg L-1 2,4-D (immature seeds and leaves) or 0.1 mg L-1 NAA (roots), explants produced embryogenic calli which upon transfer to plant growth regulator-free medium allowed embryo conversion into plantlets. The best embryogenic response (82%) was obtained in calli derived from leaves cultured with their abaxial surface in contact with medium, whereas the highest embryo conversion rate (68%) was recorded for calli induced on immature seed explants. Histological analysis in all explant types revealed development of proembryogenic cell complexes at callus periphery, giving rise to somatic embryos. The presence of embryos at various stages of development indicated asynchronous somatic embryogenesis in G. utriculosa. Derooted embryo-derived plantlets placed on medium with 0.2 mg L-1 BA multiplied further as shoot cultures.
dc.publisherDe Gruyter Open Ltd
dc.rightsrestrictedAccess
dc.sourceBiologia
dc.subjectGentiana utriculosa
dc.subjecthistology
dc.subjectimmature seeds
dc.subjectmicropropagation
dc.subjectsomatic embryogenesis
dc.titleSomatic embryogenesis and in vitro shoot propagation of Gentiana utriculosa
dc.typearticleen
dc.rights.licenseARR
dcterms.abstractУзелац, Бранка; Крстић-Милошевић, Дијана; Винтерхалтер, Драган; Митић, Невена; Винтерхалтер, Бранка;
dc.rights.holder© 2016 Institute of Botany, Slovak Academy of Sciences
dc.citation.issue2
dc.citation.volume71
dc.identifier.doi10.1515/biolog-2016-0020
dc.identifier.scopus2-s2.0-84964940279
dc.identifier.wos000374064100005
dc.citation.spage139
dc.citation.epage148
dc.type.versionpublishedVersionen
dc.citation.rankm23


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