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dc.creatorSavić, Jelena
dc.creatorDragićević, Ivana
dc.creatorPantelić, D.
dc.creatorOljača, Jasmina
dc.creatorMomčilović, Ivana D.
dc.date.accessioned2015-08-28T10:26:51Z
dc.date.available2015-08-28T10:26:51Z
dc.date.issued2012sr
dc.date.issued2012
dc.identifier.issn0354-4664sr
dc.identifier.otherRad_konverzija_358sr
dc.identifier.urihttp://ibiss-r.rcub.bg.ac.rs/123456789/298
dc.description.abstractWe have examined the correlation between heat tolerance and small heat shock protein (sHSP) expression under heat stress conditions in potato (Solanum tuberosum L.). The relative heat tolerance of nine potato cultivars grown under greenhouse conditions was determined using the electrolyte leakage assay (ELA), a standard quantitative assay for heat tolerance. Three cultivars differing in heat tolerance were selected and designated as heat-tolerant (‘Laura’), moderately sensitive (‘Liseta’) and heat-sensitive (‘Agria’) genotypes. The expression of cytosolic HSP18 and chloroplast HSP21 was analyzed at the protein level in the leaves of selected cultivars, both ex vitro- and in vitro-grown, after heat stress or control treatment. Immunoblot analysis revealed heat-induced HSP18 and HSP21 expression in all examined genotypes. A similar pattern of examined sHSP expression was observed ex vitro and in vitro: heat-tolerant ‘Laura’ accumulated higher levels of both HSP18 and HSP21 compared to heat-sensitive ‘Liseta’ and ‘Agria’. Our results indicate that ELA combined with immunoblot analysis of sHSP accumulation under HS conditions, might be considered as a reliable procedure in screening potato genotypes for heat tolerance. To our knowledge, this is the first study where sHSP expression between ex vitro- and in vitro-grown potato plants was compared.en
dc.description.sponsorshipProjekat ministarstva br. TR31049sr
dc.language.isoengsr
dc.rightsopenAccesssr
dc.sourceArchives of Biological Sciencessr
dc.subjectheat stressENG
dc.subjectheat toleranceENG
dc.subjectpotatoENG
dc.subjectsmall heat shock proteinsENG
dc.subjectsHSP electrolyte leakage assayENG
dc.titleExpression of small heat shock proteins and heat tolerance in potato (Solanum tuberosum L.)en
dc.typearticlesr
dcterms.abstractСавић, Јелена; Пантелић, Д.; Момчиловић, Ивана Д.; Драгићевић, Ивана; Ољача, Јасмина;
dc.citation.issue1sr
dc.citation.volume64sr
dc.citation.spage135sr
dc.citation.epage144sr
dc.type.versionpublishedVersionsr
dc.identifier.fulltexthttp://ibiss-r.rcub.bg.ac.rs/bitstream/handle/123456789/298/Rad_konverzija_358.pdf?sequence=1&isAllowed=y


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