Lipopolysaccharide induces tumor necrosis factor receptor-1 independent relocation of lymphocytes from the red pulp of the mouse spleen
Authors:Lalić, Ivana M.
Despotović, Sanja Z.
Milićević, Novica M.
Article (Published version)
© 2018 Elsevier GmbH
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It is well known that bacterial lipopolysaccharide (LPS) induces migration of several cellular populations within the spleen. However, there are no data about the impact of LPS on B and T lymphocytes present in the red pulp. Therefore, we used an experimental model in which we tested the effects of intravenously injected LPS on the molecular, cellular and structural changes of the spleen, with special reference to the red pulp lymphocytes. We discovered that LPS induced a massive relocation of B and T lymphocytes from the splenic red pulp, which was independent of the tumor necrosis factor receptor-1 signaling axis. Early after LPS treatment, quantitative real-time PCR analysis revealed the elevated levels of mRNA encoding numerous chemokines and proinflammatory cytokines (XCL1, CXCL9, CXCL10, CCL3, CCL4, CCL5, CCL17, CCL20, CCL22, TNFα and LTα) which affect the navigation and activities of B and T lymphocytes in the lymphoid tissues. An extreme increase in mRNA levels for CCL20 was detected in the white pulp of the LPS-treated mice. The CCL20-expressing cells were localized in the PALS. Some smaller CCL20-expressing cells were evenly dispersed in the B cell zone. Thus, our study provides new knowledge of how microbial products could be involved in shaping the structure of lymphatic organs.
Keywords:Chemokine; Lipopolysaccharide; Lymphocyte; Mouse; Red pulp; Spleen
Source:Annals of anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft, 2018, 216, 125-134
- Molecular regulation of structural organization of lymphatic organs (RS-175005)
- Alexander von Humboldt-Foundation, Bonn, Germany (Institutional Academic Cooperation between Beograd and Lübeck; DEU 1033146)
- European Union through the European Regional Development Fund (Project No. 2014-2020.4.01.15-0012)
- Estonian Research Council grant IUT2-2