Specifični obrazac ekspresije HO-1 i HO-2 u pankreasu pacova - uloga u eksperimentalno indukovanom diabetes mellitusu
Specific expressional pattern of HO-1 and HO-2 in rat pancreas: Role in experimentally induced diabetes mellitus
Buzadžić, Biljana J.
Article (Published version)
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The goal of this study was to elucidate expression of heme oxygenase inducible (HO-1) and constitutive (HO-2) isoforms in normal and diabetic pancreas and possible role of their catalytic product - carbon monoxide (CO) in this tissue. The male Mill Hill hybrid hooded rats were divided into two groups: diabetic and non-diabetic. The single dose of alloxan (120 mg/kg) was used for induction of insulin-dependent diabetes mellitus. Both groups were additionally separated in three subgroups: one, treated 12 days with L-arginine HCl (2.25%), another with Nω-nitro-L-arginine methyl ester (L-NAME HCl, 0.01%) and the third was control. HO isoforms were detected by immunohistochemistry. Strong HO-2 immunopositivity occurred in endocrine pancreas of non-diabetic control rats while weaker staining with HO-1 antibody was detected in this group. In diabetic control, as well as in non-diabetic and diabetic treated groups, isoforms specific changes of immunopositivity were observed. Regardless of group, strong immunoreactivity of one isoform was associated with weaker immunoreactivity of another. In conclusion, there is an inverse expressional pattern of HO-1 and HO-2 in normal as well as in diabetic pancreas, which enables maintaining of HO catalytic activity in this tissue. These results suggest that HO/CO pathway might be of importance for pancreatic function.