Sadržaj diarilheptanoida u kori crne (Alnus glutinosa (L.) Gaertn.) i sive jove (Alnus incana (L.) Moench) kao indikator taksonomske i populacione varijabilnosti
Content of diarylheptanoids in the bark of black (Alnus glutinosa (L.) Gaertn.) and grey alder (Alnus incana (L.) Moench) as an indicator of taxonomic and population variability
Doctoral thesis (Published version)
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In this study, two populations of Alnus glutinosa (L.) Gaertn. (Prilički kiseljak and Rimski most) and two populations of Alnus incana (L.) Moench (Golijska reka and Sastavci) were sampled on mountain Golija with the aims to investigate: 1) variability and relations of populations within and among species based on the concentration of diarylheptanoids in the bark of natural populations of A. glutinosa and A. incana; 2) seasonal variability of diarylheptanoids in the bark of A. glutinosa and A. incana; 3) the reliability of diarylheptanoids as chemotaxonomic markers in the studied species; 4) the reliability of diarylheptanoids for the detection of spontaneous hybrids between A. glutinosa and A. incana; 5) variability of leaf shape, size and venation in the populations of A. glutinosa and A. incana; 6) spontaneous hybridization between the studied species based on leaf morphology. Six diarylheptanoids (hirsutanonol-5-O-β-D-glucopyranoside, oregonin, hirsutanonol, alnuside A, platyphylloside and rubranoside A) were quantified in bark ethanol extracts of four Alnus spp. populations by UHPLC-MS/MS. Interpopulation variability of diarylheptanoid concentration was investigated with ANOVA, Kruskal-Wallis test, principal component analysis (PCA) and discriminant analysis (DA). Quantitative distribution of selected diarylheptanoids in the bark of A. glutinosa and A. incana was first analyzed in populations Rimski most and Sastavci. DA completely discriminates three groups of individuals that were implied by PCA. Based on the results, a hypothesis is proposed that the group of four individuals is of hybridogenic origin. Diarylheptanoids proved to be good chemotaxonomic markers at the interspecific level and may also reveal hybrid species. In the next vegetation season, seasonal, within population, among population and interspecies variability of diarylheptanoid concentration were investigated in two geographically close (Rimski most and Sastavci), and two relatively distant populations of black and grey alder sampled from the typical localities for the two species: Prilički kiseljak (A. glutinosa) and Golijska reka (A. incana). PCA shows clear separation of groups of individuals that belong to distant populations of A. glutinosa and A. incana, and partial overlapping of groups of individuals from geographically close populations Rimski most and Sastavci. All populations display pronounced intrapopulation variability. The most diversified is population Sastavci. Seasonal variability of diarylheptanoid content was studied in the bark sampled from populations Rimski most (A. glutinosa) and Sastavci (A. incana) in two seasons (June 2015 and April 2016). The majority of diarylheptanoids are more abundant in samples collected in June than in April. These results may indicate different plastic responses of investigated species in different seasons. The variability of leaf shape in four investigated alder populations was examined by geometric morphometry. PCA shows a continuum of leaf shapes between shapes typical for A. glutinosa and shapes typical for A. incana, with transitional shapes in the overlapping zone. These results implicate leaf morphological similarity of A. glutinosa i A. incana, great variability and phenotypic plasticity of individuals within populations and probable hybrid occurrence. Leaves from potential hybrid individuals indicated by DA have intermediate number of pairs of secondary leaf veins (SN) between A. glutinosa and A. incana. Leaf size was measured by centroid size (CS) which was significantly lower in population Sastavci (A. incana) compared to other populations; SN and CS did not show significant differences in populations of A. glutinosa. Leaf allometry, CS and SN seem to be good indicators of the influences of environmental factors.
Keywords:Alnus glutinosa; Alnus incana; Betulaceae; Diarylheptanoids; Diversity; Geometric morphometry; Chemotaxonomy; Hybridization; Morphology; Population variability
Source:Faculty of Chemistry, University of Belgrade, 2018, 1-117
APA:Vidaković, V. (2018). Content of diarylheptanoids in the bark of black (Alnus glutinosa (L.) Gaertn.) and grey alder (Alnus incana (L.) Moench) as an indicator of taxonomic and population variability. Belgrade: Faculty of Chemistry, University of Belgrade.
Vancouver:Vidaković V. Content of diarylheptanoids in the bark of black (Alnus glutinosa (L.) Gaertn.) and grey alder (Alnus incana (L.) Moench) as an indicator of taxonomic and population variability [dissertation]. Belgrade: Faculty of Chemistry, University of Belgrade; 2018. 117 p.
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