In vitro propagacija Tacitus bellus
In vitro propagation of Tacitus belhts
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Tacitus bellus Moran & J. Meyran, syn. Graptopetalum bellum, fam Crassulaceae was discovered in 1972 in a mountainous areas in Mexico and southwest of the USA. Rosettes, up to 10 cm in diameter, lie almost flat to the ground, while large clusters of star like, pink till deep red, flowers appear in May and June, which makes it one of the most fascinating new succulent plants. Sterilized lower rosette leaves were aseptically placed on medium containing, mineral and vitamin solution supplemented with sucrose (3 %), benzylaminopurine (BAP) (2.25 mg dm-3) and naphtalenacetic acid (NAA) (0.186 mg dm-3) arid gelled with agar (0.7 %), Murashige and Skoog, 1962,. Callus and a large number of buds were observed on the explants bases after six months of cultivation on the two-phase medium. The bud multiplications were performed on MS media supplemented with BAP (0.1-0.5 mg dm-3) and NAA (0.1 mg dm-3), while rooting of small rosettes were performed on NAA (0.1 - 0.2 mg dm-3). Spontaneous rooting (80 %) were noticed on hormone free medium while NAA (0.1 or 0.2 mg dm-3) stimulated it up to 100%, depending on clones. Rooted plants were easily acclimatized.