Hemijski sastav etarskog ulja Hyssopus officinalis L. i antifungalna aktivnost
Chemical composition of Hyssopus officinalis L. essential oil and its antifungal activity
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Chemical composition and antifungal activities of essential oil of Hyssopus officinalis L. and its component β-pinene were done in this paper using macrodilution and microdilution methods. Minimum inhibitory and minimum fungicidal concentrations (MIC and MFC) in ethanol and Tween were determined. Oil and component were investigated against 12 phyto-, animal- and human pathogens; Aspergillus niger, A. ochraceus, A. versicolor, A. flavus, A. terreus, Alternaria alternata, Penicillium ochrochloron, P. funiculosum, Cladosporium cladosporioides, Trichoderma viride, Fusarium tricinctum, Phomopsis helianthi. In essential oil of H. officinalis 27 components were identified (91.70% of total oil). The major compounds were isopinocamphone (43.29%), trans-pinocamphone (16.79%) and β-pinene (16.31%). The essential oil showed strong antifungal activity, with MIC of 2.5 to 3.5 ml/ml in ethanol and Tween. MIC were almost the same in micro- and macrodilution methods, except for Penicillium species where oil dissolved in Tween showed lower MIC. MFC were the same as in macrodilution method. In comparation with bifonazole, essential oil showed much better antifungal potential. .