Variranje in vitro odgovora pšenice, uticaj genotipa i spoljne sredine
Variability of in vitro culture response in wheat genotypes, genotype and environmental effects
Article (Published version)
MetadataShow full item record
The tissue culture response (TCR) of immature embryos, evaluated according to callus formation, percentage of regenerative green-spotted calli and the number of plants per embryo, was investigated in 96 wheat genotypes of worldwide origins. Immature embryos were collected 12-15 DAP from field-grown plants during three successive years 2003, 2004 and 2005. Year 2003 was with high air temperatures and tropical days during a period of vegetation, while the environmental conditions were more favorable for plant growth in the next two years, 2004 and 2005. Embryos were cultured on standard MS medium containing 2 mg l-1 2, 4-D. In all genotypes calli were efficiently induced, ranging from 36.7 to 100% (2003), 68.4 to 100% (2004), and 94.3 to 100% (2005). The calli occasionally formed green spots, but frequencies markedly differed among genotypes that varied from 0 to 72.5% (2003), 0 to 97.9% (2004), and 0 to 94.0% (2005). Coefficient of variation was highest in term of percent of regenerative calli (66.7%) following by a number of plants per embryo (35.6%) and callus formation (5.1%). Components of phenotypic variance showed that factor year (71.4%) had the highest impact on expression of callus formation, genetic factor (47,1%) on percentage of regenerative green-spotted calli and interaction year/genotype (30.3%) on number of plants per embryo. The results indicated factor genotype as the most important for determining regeneration potential in wheat.