Efekti oktreotida na ACTH ćelije hipofize ženki pacova
The effects of centrally applied octreotide on ACTH cells in the female rat
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The effects of intracerebroventricular (i.c.v.) application of a somatostatin analogue, Octreotide, on growth of pituitary adrenocorticotropes (ACTH cells) were examined in adult female Wistar rats. The animals were subjected to i.c.v. administration of three 1.0μg doses of Octreotide dissolved in 10μ1 saline every second day. Controls were treated in the same way with the same volume of saline. ACTH-producing cells were studied using the peroxidase -antiperoxidase (PAP) immunohistochemical procedure. Octreotide treatment of rat females, significantly decreased (p<0.05) all morphometric parameters measured, i.e. volume of ACTH cells and their nuclei by 52% and 11 %, respectively, as compared to the controls. The volume densities were also significantly decreased (by 47%; p<0.05) in comparison with the corresponding controls. These findings suggest that centrally administered somatostatin analogue (Octreotide) is specifically involved in the control of growth and secretory activity of ACTH cells in female rats.