Semikvantitativni citohemijski metod u evaluaciji pušenjem izazvanih promena u sadržaju glikogena alveoralnih makrofaga i njihovih apoptopskih karakteristika
Semiquantitative cytochemical method in the evaluation of smoking induced changes of alveolar macrophages glycogen and apoptotic properties
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Having in mind hypothesis about discrete changes in alveolar macrophages (AMs') biological markers under the smoking exposure, the study was designed regarding cytological, cytochemical and apoptotic parameters in lung washings. Cell profile and apoptotic capacity (AC) of pulmonary tissue evaluated by bronchoalveolar lavage (BAL) were evaluated by light microscopy in fifteen subjects: 9 non-smokers and 6 smokers. Apoptosis was detected by TUNEL in situ cytochemical method. Semiquantitative indexing and scorring methods were used for AC and evaluation of alveolar macrophages (AMs) glycogen by PAS reaction. Significant increase of macrophages, AC and decrease eosinophils (p<0.05) were revealed in smokers in comparison with nonsmokers. There is significant correlation of AMs' glycogen and smoking exposure (Spearman R= 0.98, p<0.001) as well as eosinophils and AMs' glycogen (Spearman R=0.81, p<0.05). In nonsmokers, percentage of free apoptotic bodies (FAB), correlates with amounts of glycogen in AMs (Spearman R=-0.79, p<0.05). The evidence of smoking induced changes of AMs' metabolic properties supports an idea of cell energy metabolism switching which might be important for programmed cell death regulation in order to avoid harmful influence of noxious agents, including tobacco smoke.