Inhibicija aktivnosti antioksidativnih enzima u eritrocitima pacijenata sa akutnim infarktom miokarda
Antioxidative defense enzyme inhibition in erythrocytes of patients with acute myocardial infarction
Kastratović, Dragana A.
Nikolić, Aleksandra L.
Mijalković, Dejan N.
Article (Published version)
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Increased amount of reactive oxygen and nitrogen species were detected during myocardial ischemia after acute myocardial infarction (AMI). Decreased activity of some antioxidative defense enzymes in erythrocytes creates conditions for oxidative stress and propagation of thus induced damages. In this study, we tried to detect changes in antioxidative defense enzyme activities in erythrocytes of patients with AMI and to determine a nature of this changes. Activity of copper zinc super oxide dismutase (CuZn SOD, EC 22.214.171.124), catalase (CAT, EC 126.96.36.199), glutathione peroxidase (GSH-Px, EC 188.8.131.52) and glutathione reductase (GR, EC 184.108.40.206) were determined in AMI patiens and compared with controls. Activities of CuZn SOD (p<0.005) and CAT (p<0.05), as well as, GSH-Px (p<0.02) were significantly lower comparing with controls. Activity of GR was significantly higher (p<0.005) with respect to controls. Activity of CuZn SOD in patients with AMI were more inhibited (47 ± 5 %) with 6 mM diethyldithiocarbamate (DDC) than corresponding controls (32 ± 3 %). In vitro inhibition of control samples with hydrogen peroxide (H2O2) and electrophoretic analysis indicate H2O2 as potential inhibitor of CuZn SOD in patient with AMI.