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dc.creatorMilosević, Maja S
dc.creatorPetrović, Snjezana B
dc.creatorVeličković, Nataša
dc.creatorGrković, Ivana S
dc.creatorIgnjatović, Marija
dc.creatorHorvat, Anica I
dc.date.accessioned2017-11-23T11:12:25Z
dc.date.available2015-11-17T10:26:51Z
dc.date.issued2012sr
dc.identifier.issn0300-8177sr
dc.identifier.otherRad_konverzija_3079sr
dc.identifier.urihttps://ibiss-r.rcub.bg.ac.rs/handle/123456789/1084
dc.description.abstractExtracellular nucleotides affect female reproductive functions, fertilization, and pregnancy. The aim of this study was to investigate biochemical characteristics of ATP and ADP hydrolysis and identify E-NTPDases in myometrial cell membranes from Wistar albino rats. The apparent K (m) values were 506.4 +/- A 62.1 and 638.8 +/- A 31.3 mu M, with a calculated V (max) (app) of 3,973.0 +/- A 279.5 and 2,853.9 +/- A 79.8 nmol/min/mg for ATP and ADP, respectively. The enzyme activity described here has common properties characteristic for NTPDases: divalent cation dependence; alkaline pH optimum for both substrates, insensitivity to some of classical ATPase inhibitors (ouabain, oligomycine, theophylline, levamisole) and significant inhibition by suramine and high concentration of sodium azides (5 mM). According to similar apparent K-m values for both substrates, the ATP/ADP hydrolysis ratio, and Chevillard competition plot, NTPDase1 is dominant ATP/ADP hydrolyzing enzyme in myometrial cell membranes. RT-PCR analysis revealed expression of three members of ectonucleoside triphosphate diphosphohydrolase family (NTPDase 1, 2, and 8) in rat uterus. These findings may further elucidate the role of NTPDases and ATP in reproductive physiology.en
dc.description.sponsorshipSerbian Ministry of Education and Science [173044, 41014]sr
dc.language.isoEnglishsr
dc.rightsrestrictedAccess
dc.sourceMolecular and Cellular Biochemistrysr
dc.titleATP and ADP hydrolysis in cell membranes from rat myometriumen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractВеличковић, Наташа; Петровић, Сњезана Б; Грковић, Ивана С; Игњатовић, Марија; Хорват, Aница И; Милосевић, Маја С;
dc.citation.issue1-2sr
dc.citation.volume371sr
dc.citation.spage248sr
dc.citation.epage208sr
dc.type.versionpublishedVersionen
dc.citation.rankM23


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