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dc.creatorNedeljković, Nadezda N
dc.creatorBjelobaba, Ivana
dc.creatorLavrnja, Irena
dc.creatorStojkov, Danijela
dc.creatorPeković, Sanja
dc.creatorRakić, Ljubisav
dc.creatorStojiljković, Mirjana B
dc.date.accessioned2017-11-23T11:12:53Z
dc.date.available2015-11-17T10:26:51Z
dc.date.issued2008sr
dc.identifier.issn0364-3190sr
dc.identifier.otherRad_konverzija_3528sr
dc.identifier.urihttps://ibiss-r.rcub.bg.ac.rs/handle/123456789/1533
dc.description.abstractDuring a variety of insults to the brain adenine nucleotides are released in large quantities from damaged cells, triggering multiple cellular responses to injury. Here, we evaluated changes in extracellular ATP, ADP and AMP hydrolysis at different times (0-24 hours) after unilateral cortical stab injury (CSI) in adult rats. Results demonstrated that 24 hours following CSI, ATP and ADP hydrolyzing activities were not significantly altered in injured cortex. Based on calculated V (ATP)/V (ADP) ratio it was concluded that ATP/ADP hydrolysis was primarily catalyzed by NTPDase1 enzyme form. In contrast, AMP hydrolysis, catalyzed by 5'-nucleotidase, was significantly reduced at least 4 hours following CSI. Kinetic analysis and Lineweaver-Burk transformation of the enzyme velocities obtained over the range of AMP concentrations (0.05-1.50 mM) revealed that inhibition of 5'-nucleotidase activity after CSI was of the uncompetitive type. Taken together our data suggest that injured tissue has reduced potential for extracellular metabolism of adenine nucleotides in early stages after CSI.en
dc.description.sponsorshipnullsr
dc.language.isoEnglishsr
dc.rightsrestrictedAccess
dc.sourceNeurochemical Researchsr
dc.titleEarly temporal changes in ecto-nucleotidase activity after cortical stab injury in raten
dc.typearticle
dc.rights.licenseARR
dcterms.abstractНедељковић, Надезда Н; Стојков, Данијела; Ракић, Љубисав; Пековић, Сања М.; Бјелобаба, Ивана; Лаврња, Ирена Ц.; Стојиљковић, Мирјана Б;
dc.citation.issue5sr
dc.citation.volume33sr
dc.citation.epage879sr
dc.type.versionpublishedVersionen
dc.citation.rankM23


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