Dinamičke asocijacije transkripcionih faktora sa nuklearnim matriksom jetre pacova su funkcionalno povezane sa diferencijalnom genskom ekspresijom alfa-2-makroglobulina
Dynamic associations of transcription factors with the rat liver nuclear matrix are functionally related to differential alpha-2-macroglobulin gene expression
Ivanović Matić, Svetlana
Article (Published version)
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Participation of the nuclear matrix in regulation of alpha-2-macroglobulin (α2M) gene transcription during rat liver development and the acute-phase (AP) response are examined. DNA affinity chromatography of fetal and adult liver internal nuclear matrix proteins under basal and AP conditions with the α2M gene promoter (-852/+12) and immunoblot analysis revealed diverse patterns of association of transcription factors with the nuclear matrix. HNF-6, C/EBPα, and STAT5b were involved in basal and C/EBPβ, STAT1, and STAT3 in AP-stimulated α2M expression. These findings support the assumption that transcription factor-nuclear matrix interactions serve to channel gene regulatory proteins to DNA sequences.
Keywords:Alpha-2-macroglobulin; acute phase response; rat liver; nuclear matrix; transcription factors
Source:Archives of Biological Sciences, 2008, 60, 3, 355-366