Response of alpha-glucosidase in gypsy moth larvae to acute and chronic dietary cadmium
Perić Mataruga, Vesna
Article (Published version)
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We investigated the effects of acute and chronic treatments with cadmium at 10 mu g Cd/g dry food and 30 mu g Cd/g dry food on alpha-glucosidase activity of the 4th instar larvae of Lymantria dispar (Lepidoptera: Lymantriidae) as well as subsequent recovery. Enzyme inhibition was recorded during acute exposure to 30 mu g Cd/g dry food and during chronic treatment at the lower metal concentration. After three days recovery from 10 mu g Cd/g dry food, the alpha-glucosidase activity returned to the control level. One-way ANOVA showed that cadmium significantly influenced the activity of alpha-glucosidase during all treatments. The index of phenotypic plasticity was higher during chronic treatment at 10 mu g Cd/g dry food than at 30 mu g Cd/g as well as during the recovery. We detected four glucosidase isoforms by NATIVE PAGE. The activities and expressions of the isoforms depended on both larval genotype and cadmium treatment.