Karakterizacija endopeptidaza srednjeg creva larvi Morimus funereus (Coleoptera: Cerambycidae)
Characterization of endopeptidases from the midgut of Morimus funereus (Coleoptera: Cerambycidae) larvae
Vujčić, Zoran M.
Article (Published version)
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Application of specific chromogenic substrates, use of class-specific inhibitors, and zymogram analysis enabled us to identify several peptidase classes in extracts of the midgut of Morimus funereus larvae. Zymogram analysis with gelatin as a peptidase substrate and phenylmethylsulfonyl fluoride as an inhibitor showed that serine peptidases were the most abundant endopeptidases in the midgut of M. funereus larvae. By zymogram analysis with gelatin as a peptidase substrate and 1,10-phenanthroline as an inhibitor, metallopeptidases were also detected. Analyses of serine peptidases with specific chromogenic substrates revealed dominance of elastase-like peptidases in extracts of the midgut of M. funereus larvae, with less pronounced chymotrypsin- and trypsin-like activities.
Keywords:Cerambycid beetle; Morimus funereus; midgut peptidases; protease inhibitors; synthetic substrate; zymogram
Source:Archives of Biological Sciences, 2008, 60, 3, 403-409