Show simple item record

The mechanism of cytotoxic and cytoprotective effects of fullerene (C60) nanoparticles

dc.contributor.advisorTrajković, Vladimir
dc.contributor.otherIsaković, Aleksandra
dc.contributor.otherMarković, Zoran
dc.contributor.otherVolarević, Vladislav
dc.creatorMisirkić Marjanović, Maja
dc.date.accessioned2017-11-23T08:23:59Z
dc.date.available2017-11-23T08:23:59Z
dc.date.issued2015
dc.identifier.urihttp://eteze.bg.ac.rs/application/showtheses?thesesId=2707
dc.identifier.urihttps://fedorabg.bg.ac.rs/fedora/get/o:10829/bdef:Content/download
dc.identifier.urihttp://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=512260532
dc.identifier.urihttp://nardus.mpn.gov.rs/123456789/5056
dc.identifier.urihttps://ibiss-r.rcub.bg.ac.rs/handle/123456789/2437
dc.description.abstractU ovoj doktorskoj disertaciji ispitivani su citotoksični efekti koloidnih rastvorafulerenskih nanočestica, kao i uticaj mehanohemijski pripremljenih fulerena nacitotoksičnost azot monoksida. Zbog sposobnosti da indukuju ćelijsku smrt u određenimuslovima, fulereni C60 predstavljaju potencijalne antitumorske i toksične agense.Koloidni rastvor kristalnog C60 (nC60) je izuzetno toksičan, ali mehanizmi njegovecitotoksičnosti još uvek nisu dovoljno ispitani ni razjašnjeni. U ovoj studiji korišćena sutri različita načina pripreme nC60 rastvora: 1. metodom izmene rastvarača utetrahidrofuranu (THF/nC60), 2. etanolu (EtOH/nC60) ili 3. produženim mešanjem u vodi(H2O/nC60). Kombinovanjem eksperimentalnih analiza i matematičkog modelovanjaispitivani su uslovi pod kojim različiti rastvori nC60 ispoljavaju citotoksičnostposredovanu reaktivnim kiseoničkim vrstama (RKV). Prema sposobnosti da generišuRKV i izazovu mitohondrijalnu depolarizaciju praćenu nekrozom, rastvori nC60 surangirani sledećim redosledom: THF/nC60 > EtOH/nC60 > H2O/nC60. Matematičkomodelovanje produkcije singletnog kiseonika (1O2) pokazuje da sposobnost rastvaračaugrađenog u strukturu fulerenskog kristala da neutrališe 1O2 (THF/nC60 < EtOH/nC60 <H2O/nC60) predstavlja presudni faktor koji određuje sposobnost nanočestice daproizvodi RKV i izaziva ćelijsko oštećenje. Ovi rezultati skreću pažnju na toksikološkiaspekt koloidnih fulerena u njihovoj potencijalnoj upotrebi u biomedicini.Fulereni imaju dvojnu prirodu tako da osim što proizvode RKV kada supobuđeni vidljivom svetlošću, oni deluju i kao ,,skupljači“ slobodnih radikala iispoljavaju značajna antioksidativna svojstva. Iz tog razloga je, u drugom delu studije,ispitivan uticaj fulerenskih nanočestica na citotoksičnost azot monoksida (NO), veomareaktivnog slobodnog radikala. Fulerenske nanočestice su pripremljene mehanohemijskipotpomognutom kompleksacijom uz korišćenje anjonskog surfaktanta natrijum dodecilsulfata (SDS), makrocikličnog oligosaharidγa -ciklodekstrina (γCDX) ili kopolimeraacetat-etilen vinil versatata (EVA-EVV) i karakterisane metodama UV-visspektroskopije i mikroskopijom atomskih sila (AFM)...sr
dc.description.abstractIn this doctoral dissertation we investigated cytotoxic effects of fullerene colloidalsuspensions, as well as the influence of mechanochemically synthesized fullerene C60nanoparticles on the cytotoxicity of nitric oxide (NO). Because of their ability to inducecell death in certain conditions, fullerenes are potential toxic and anticancer agents.Colloidal suspension of crystalline C60 (nC60) is very toxic, but the mechanisms of itscytotoxicity are not completely explained. By combining experimental analysis andmathematical modeling, the requirements for the reactive oxygen species (ROS)-mediated cytotoxicity of different nC60 suspensions were investigated. Colloidalsuspensions of nC60 were prepared by solvent exchange method in tetrahydrofuran(THF/nC60) and ethanol (EtOH/nC60), or by extended mixing in water (H2O/nC60). Withregard to their capacity to generate RKV and cause mitochondrial depolarizationfollowed by necrotic cell death, the nC60 suspensions were ranked in the followingorder: THF/nC60 > EtOH/nC60 > aqu/nC60. Mathematical modeling of singlet oxygen(1O2) generation indicates that the 1O2-quenching power (THF/ nC60 < EtOH/nC60<H2O/nC60) of the solvent intercalated in the fullerene crystals determines their abilityto produce ROS and cause cell damage. These results could have important implicationsfor the toxicology and biomedical application of colloidal fullerenes.Fullerenes are molecules with dual properties, able to produce ROS whenphotoexcited, but also to scavenge free radicals and exert antioxidant action. In order toassess the latter, the influence of fullerene C60 nanoparticles on the cytotoxicity of ahighly reactive free radical nitric oxide (NO) was investigated. Fullerene nanoparticleswere prepared by mechanochemically assisted complexation with anionic surfactantsodium dodecyl sulfate, macrocyclic oligosaccharide γ-cyclodextrin or the copolymerethylene vinyl acetate–ethylene vinyl versatate, and characterized by UV–vis andatomic force microscopy (AFM)...en
dc.formatapplication/pdf
dc.languagesr
dc.publisherBelgrade: University of Belgrade, School of Medicinesr
dc.rightsopenAccess
dc.sourceUniversity of Belgrade, School of Medicinesr
dc.subjectNanočesticesr
dc.subjectCitotoksičnostsr
dc.subjectSlobodni radikalisr
dc.subjectAzot monoksidsr
dc.subjectApoptozasr
dc.subjectNanoparticlesen
dc.subjectCytotoxicityen
dc.subjectFree radicalsen
dc.subjectNitric oxideen
dc.subjectApoptosisen
dc.titleMehanizmi citotoksičnog i citoprotektivnog dejstva fulerenskih (C60) nanočesticasr
dc.titleThe mechanism of cytotoxic and cytoprotective effects of fullerene (C60) nanoparticlesen
dc.typedoctoralThesis
dc.rights.licenseBY-NC-ND
dcterms.abstractТрајковић, Владимир; Исаковић, Aлександра; Марковић, Зоран; Воларевић, Владислав; Мисиркић Марјановић, Маја; Механизми цитотоксичног и цитопротективног дејства фулеренских (Ц60) наночестица; Механизми цитотоксичног и цитопротективног дејства фулеренских (Ц60) наночестица;
dc.citation.apaMisirkić Marjanović, M. (2015). Mehanizmi citotoksičnog i citoprotektivnog dejstva fulerenskih (C60) nanočestica. University of Belgrade, School of Medicine.
dc.citation.vancouverMisirkić Marjanović M. Mehanizmi citotoksičnog i citoprotektivnog dejstva fulerenskih (C60) nanočestica [dissertation]. Belgarde: University of Belgrade, School of Medicine; 2015. 79 p.
dc.citation.spage1
dc.citation.epage79
dc.type.versionpublishedVersionen
dc.identifier.fulltexthttp://ibiss-r.rcub.bg.ac.rs//bitstream/id/232/Misirkic_Marjanovic_Maja_dissertation.pdf


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record