Ekspresija, produkcija i moguća uloga interleukina 10 i faktora nekroze tumora tokom eksperimentalnog autoimunskog encefalomijelitisa u pacova sojeva AO i DA
Experession, production and possible role of interleukin 10 and tumor necrosis factor during experimental autoimmune encephalomyelitis in AO and DA rats
Doctoral thesis (Published version)
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Multiple sclerosis (MS) is a chronic inflammatory and neurodegenerative disease of thecentral nervous system (CNS) with a proposed autoimmune pathogenesis. It mainlyaffects population between 20-40 years of age, more often women in comparison to menAlso, it is more often in Europe and north America comparing to other parts of theworld.. The cause of this disease is still not well defined, but it is thought to be complexinteraction between genes and different environmental factors.Experimental autoimmune encephalomyelitis (EAE) is the most commonly used MSanimal model. It is mostly induced in rodents by using spinal cord homogenate, myelinor some of the myelin’s proteins and mixing them with adjuvants. After theimmunization antigen specific CD4+ T cells proliferate and differentiate in draininglymph nodes after which they migrate and spread all around the body. One part of theantigen specific cells goes to CNS, crosses blood-brain barrier and enters theperivascular space where it recognizes antigens which they are specific for. Afterencountering the antigens T cells are reactivated and they start producing cytokines,activate local cells and attract other inflammatory cells in to the CNS. This is howinflammatory reaction begins leading to distraction of myelin sheet or even axons insome cases, finally causing neurodegeneration.Cytokines are important mediators of every inflammatory process. IL-10 is an antiinflammatorycytokine with a primary role in tissue protection and restriction ofinflammatory response. On the other hand, TNF is a classical pro-inflammatorycytokine which mediates acute and chronic systemic inflammatory reaction. The role ofboth these cytokines has been well studied in EAE model, where IL-10 is considered tobe protective while TNF is mostly thought of as deleterious and responsible forincreasing the inflammatory reaction...
Keywords:Experimental autoimmune encephalomyelitis; IL-10; TNF; CXCL12; Central nervous system; CD4+ cells; Astrocytes
Source:University of Belgrade, Faculty of Biology, 2014, 1-141
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