Show simple item record

dc.creatorJarić, Ivana
dc.creatorŽivanović, Jasmina
dc.creatorMiler, Marko
dc.creatorAjdžanović, Vladimir
dc.creatorBlagojević, Duško
dc.creatorRistić, Nataša
dc.creatorMilošević, Verica
dc.creatorNestorović, Nataša
dc.date.accessioned2017-12-25T13:39:55Z
dc.date.available2900-01-01
dc.date.issued2018
dc.identifier.issn0041-008X
dc.identifier.urihttp://linkinghub.elsevier.com/retrieve/pii/S0041008X1730474X
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/pubmed/29217487
dc.identifier.urihttps://ibiss-r.rcub.bg.ac.rs/handle/123456789/2942
dc.description.abstractThis study aimed to investigate the effects of soy isoflavones, genistein (GEN) and daidzein, (DAI) on the uterine function in ovary-intact middle-aged rats. GEN and DAI (35mg/kg) were subcutaneously administrated to acyclic (12-month-old) Wistar females, daily, for 4weeks. Control group received either vehicle (olive oil and ethanol, 9:1) or remained intact. We found that GEN and DAI differently affect uterine morphophysiology. GEN significantly increased the uterine wet weight which was associated with hyperplastic changes, revealed by stereological and histomorphometrical analyses. Also, PCNA immunoexpression was increased, whereas expression of apoptotic marker (caspase-3) was decreased. Protein and gene expressions of ERα were down-regulated, while PR and ERβ were up-regulated after GEN application. Also, GEN caused an increase of LAC and VEGF mRNA expression, together with an up-regulation of Akt activity. In contrast, DAI did not change the uterine wet weight and stereological features of the main uterine compartments as well as LAC and VEGF gene expression. Absence of hyperplastic changes were illustrated by an increase in caspase-3 immunoexpression, associated with reduced PCNA expression. DAI up-regulated only the expression of ERβ, while the expression levels of ERα and PR remain unaffected. Also, DAI inhibited the activation of Akt due to down-regulation of phosphorylated and total form of Akt protein expression. Compared to GEN, DAI did not promote events associated with the endometrial cell proliferation in the conducted study, figuring as the compound with a potential safety profile, which justifies further investigation.
dc.relationCOST Action FA 1403 POSITIVe (Interindividual variation in response to consumption of plant food bioactives and determinants involved)
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173009/RS//
dc.rightsrestrictedAccess
dc.sourceToxicology and Applied Pharmacology
dc.sourceToxicology and Applied Pharmacology
dc.subjectIsoflavones
dc.subjectMiddle-aged rats
dc.subjectOvary-intact
dc.subjectProliferation
dc.subjectSteroid receptors
dc.subjectUterine function
dc.titleGenistein and daidzein treatments differently affect uterine homeostasis in the ovary-intact middle-aged rats.
dc.typearticleen
dc.rights.licenseARR
dcterms.abstractМилошевић, Верица; Несторовић, Наташа; Ристић, Наташа; Благојевић, Душко; Aјджановић, Владимир; Милер, Марко; Јарић, Ивана; Живановић, Јасмина;
dc.rights.holder© 2017 Elsevier Inc
dc.citation.volume339
dc.identifier.doi10.1016/j.taap.2017.12.001
dc.identifier.pmid29217487
dc.identifier.scopus2-s2.0-85037620773
dc.identifier.wos000423781600008
dc.citation.apaJarić, I., Živanović, J., Miler, M., Ajdžanović, V., Blagojević, D., Ristić, N., Milošević, V., et al. (2018). Genistein and daidzein treatments differently affect uterine homeostasis in the ovary-intact middle-aged rats. Toxicology and Applied Pharmacology, 339, 73–84.
dc.citation.vancouverJarić I, Živanović J, Miler M, Ajdžanović V, Blagojević D, Ristić N, Milošević V, Nestorović N. Genistein and daidzein treatments differently affect uterine homeostasis in the ovary-intact middle-aged rats. Toxicol Appl Pharmacol. 2018;339:73–84.
dc.citation.spage73
dc.citation.epage84
dc.type.versionpublishedVersionen
dc.citation.rankM21


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record