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dc.creatorOreščanin Dušić, Zorana
dc.creatorTatalović, Nikola
dc.creatorVidonja Uzelac, Teodora
dc.creatorBrkljačić, Jelena
dc.creatorNikolić-Kokić, Aleksandra
dc.creatorMijušković, Ana
dc.creatorSpasić, Mihajlo
dc.creatorPaškulin, Roman
dc.creatorBresjanac, Mara
dc.creatorBlagojević, Duško
dc.date.accessioned2018-02-12T09:06:57Z
dc.date.available2018-02-12T09:06:57Z
dc.date.issued2018
dc.identifier.urihttps://www.hindawi.com/journals/omcl/2018/5969486/
dc.identifier.urihttps://ibiss-r.rcub.bg.ac.rs/handle/123456789/2970
dc.description.abstractIbogaine is an indole alkaloid originally extracted from the root bark of the African rainforest shrub Tabernanthe iboga. It has been explored as a treatment for substance abuse because it interrupts drug addiction and relieves withdrawal symptoms. However, it has been shown that ibogaine treatment leads to a sharp and transient fall in cellular ATP level followed by an increase of cellular respiration and ROS production. Since contractile tissues are sensitive to changes in the levels of ATP and ROS, here we investigated an ibogaine-mediated link between altered redox homeostasis and uterine contractile activity. We found that low concentrations of ibogaine stimulated contractile activity in spontaneously active uteri, but incremental increase of doses inhibited it. Inhibitory concentrations of ibogaine led to decreased SOD1 and elevated GSH-Px activity, but doses that completely inhibited contractions increased CAT activity. Western blot analyses showed that changes in enzyme activities were not due to elevated enzyme protein concentrations but posttranslational modifications. Changes in antioxidant enzyme activities point to a vast concentration-dependent increase in H2O2 level. Knowing that extracellular ATP stimulates isolated uterus contractility, while H2O2 has an inhibitory effect, this concentration-dependent stimulation/inhibition could be linked to ibogaine-related alterations in ATP level and redox homeostasis.
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173014/RS//
dc.relationSlovenian ARRS Programme P3-0171
dc.relationSerbian-Slovenian bilateral cooperation “Integrative approach to basic housekeeping metabolism and adaptive plasticity screening in different ex vivo tissue models”
dc.rightsopenAccess
dc.sourceOxidative Medicine and Cellular Longevity
dc.titleThe Effects of Ibogaine on Uterine Smooth Muscle Contractions: Relation to the Activity of Antioxidant Enzymes
dc.typearticleen
dc.rights.licenseBY
dcterms.abstractВидоња Узелац, Теодора; Мијушковић, Aна; Несторов, Јелена; Таталовић, Никола; Николић-Кокић, Aлександра; Спасић, Михајло; Пашкулин, Роман; Бресјанац, Мара; Благојевић, Душко; Орешчанин Душић, Зорана;
dc.rights.holder© 2018 Zorana Oreščanin-Dušić et al
dc.citation.volume2018
dc.identifier.doi10.1155/2018/5969486
dc.identifier.pmid29599898
dc.identifier.scopus2-s2.0-85052803886
dc.identifier.wos000425832500001
dc.citation.apaOreščanin-Dušić, Z., Tatalović, N., Vidonja-Uzelac, T., Nestorov, J., Nikolić-Kokić, A., Mijušković, A., Spasić, M., et al. (2018). The Effects of Ibogaine on Uterine Smooth Muscle Contractions: Relation to the Activity of Antioxidant Enzymes. Oxidative Medicine and Cellular Longevity, 2018, 5969486.
dc.citation.vancouverOreščanin-Dušić Z, Tatalović N, Vidonja-Uzelac T, Nestorov J, Nikolić-Kokić A, Mijušković A, Spasić M, Paškulin R, Bresjanac M, Blagojević D. The Effects of Ibogaine on Uterine Smooth Muscle Contractions: Relation to the Activity of Antioxidant Enzymes. Oxid Med Cell Longev. 2018;2018:5969486.
dc.citation.spage5969486
dc.type.versionpublishedVersionen
dc.identifier.fulltexthttp://ibiss-r.rcub.bg.ac.rs//bitstream/id/4100/OxidMedCellLongev_2018_2018_5969486.pdf


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