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dc.creatorMatić, Sanja
dc.creatorStanić, Snežana
dc.creatorBogojević, Desanka
dc.creatorVidaković, Melita
dc.creatorGrdović, Nevena
dc.creatorDinić, Svetlana
dc.creatorSolujić, Slavica
dc.creatorMladenović, Milan
dc.creatorStanković, Nevena
dc.creatorMihailović, Mirjana
dc.date.accessioned2018-11-26T14:10:57Z
dc.date.available2018-11-26T14:10:57Z
dc.date.issued2013
dc.identifier.urihttp://www.sciencedirect.com/science/article/pii/S1383571813001836
dc.identifier.urihttps://ibiss-r.rcub.bg.ac.rs/handle/123456789/3188
dc.description.abstractThe present study was undertaken to investigate the hepatoprotective effect of the methanol extract of Cotinus coggygria Scop. in rats exposed to the hepatotoxic compound pyrogallol. Assessed with the alkaline version of the comet assay, 1000 and 2000. mg/kg body weight (bw) of the extract showed a low level of genotoxicity, while 500. mg/kg bw of the extract showed no genotoxic potential. Quantitative HPLC analysis of phenolic acids and flavonoids in the methanol extract of C. coggygria showed that myricetin was a major component. To test the hepatoprotective effect, a non-genotoxic dose of the C. coggygria extract and an equivalent amount of synthetic myricetin, as present in the extract, were applied either 2 or 12. h prior to administration of 100. mg/kg bw of pyrogallol. The extract and myricetin promoted restoration of hepatic function by significantly reducing pyrogallol-induced elevation in the serum enzymes AST, ALT, ALP and in total bilirubin. As measured by the decrease in total score and tail moment, the DNA damage in liver was also reduced by the extract and by myricetin. Our results suggest that pro-surviving Akt activity and STAT3 protein expression play important roles in decreasing DNA damage and in mediating hepatic protection by the extract. These results suggest that myricetin, as a major component in the extract, is responsible for the antigenotoxic and hepatoprotective properties of the methanol extract of C. coggygria against pyrogallol-induced toxicity. © 2013 Elsevier B.V.
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173020/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43004/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43010/RS//
dc.rightsrestrictedAccess
dc.sourceMutation Research - Genetic Toxicology and Environmental Mutagenesis
dc.sourceMutation Research - Genetic Toxicology and Environmental Mutagenesis
dc.subjectAkt
dc.subjectCotinus coggygria extract
dc.subjectHepatoprotection
dc.subjectMyricetin
dc.subjectPyrogallol
dc.subjectSTAT3
dc.titleMethanol extract from the stem of Cotinus coggygria Scop., and its major bioactive phytochemical constituent myricetin modulate pyrogallol-induced DNA damage and liver injury
dc.typearticleen
dc.rights.licenseARR
dcterms.abstractСтанковић, Невена; Матић, Сања; Станић, Снежана; Богојевић, Десанка; Видаковић, Мелита; Грдовић, Невена; Динић, Светлана; Солујић, Славица; Младеновић, Милан; Михаиловић, Мирјана;
dc.rights.holder© 2013 Elsevier B.V.
dc.citation.issue2
dc.citation.volume755
dc.identifier.doi10.1016/j.mrgentox.2013.03.011
dc.identifier.pmid23830930
dc.identifier.scopus2-s2.0-84881023344
dc.identifier.wos000323630100001
dc.citation.spage81
dc.citation.epage89
dc.type.versionpublishedVersionen


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