Comparison of dendritic cells obtained from autoimmunty-prone and resistant rats.
Mansilla, M. José
Mostarica Stojković, Marija
Članak u časopisu (Recenzirana verzija)
MetapodaciPrikaz svih podataka o dokumentu
Dendritic cells (DC) are responsible for the initiation and shaping of the adaptive immune response and are in the focus of autoimmunity research. We were interested in comparison of DC obtained from autoimmunity-prone Dark Agouti (DA) rats and autoimmunity-resistant Albino Oxford (AO) rats. DC were generated from bone marrow precursors and matured (mDC) by lipopolysaccharide. Tolerogenic DC (tolDC) obtained by vitamin D3 treatment were studied in parallel. Profile of cytokine production was different in AO and DA mDC and tolDC. Expression of MHC class II molecules and CD86 were higher in DA DC, while vitamin D3 reduced their expression in dendritic cells of both strains. Allogeneic proliferation of CD4+ T cells was reduced by AO tolDC, but not with DA tolDC in comparison to respective mDC. Finally, expression of various genes identified as differentially expressed in human mDC and tolDC was also analyzed in AO and DA DC. Again, AO and DA DC differed in the expression of the analyzed genes. To conclude, AO and DA DC differ in production of cytokines, expression of antigen presentation-related molecules and in regulation of CD4+ T proliferation. The difference is valuable for understanding the divergence of the strains in their susceptibility to autoimmunity.
Ključne reči:Albino Oxford rats; Allogeneic proliferation; Autoimmunity; Dark agouti rats; Dendritic cells
- Ćelijski i molekulski mehanizmi oporavka pacova od eksperimentalnog autoimunskog encefalomijelitisa (RS-173035)
- Molekularni mehanizmi fiziološke i farmakološke kontrole inflamacije i kancera (RS-173013)
- projects PI14/01175 and PI17/01521
- Short Term Scientific Missions through A FACTT network (Cost Action BM1305: www.afactt.eu)