Up-regulation of ectonucleotidase activity after cortical stab injury in rats
Article (Published version)
© 2006 International Federation for Cell Biology
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The objective of this study was to examine the changes in the activity and expression of ectonucleotidase enzymes in the model of unilateral cortical stab injury (CSI) in rat. The activities of ecto-nucleoside triphosphate diphosphohydrolase 1 (NTPDase 1) and ecto 50-nucleotidase were assessed by measuring the levels of ATP, ADP and AMP hydrolysis in the crude membrane preparations obtained from injured left cortex, right cortex, left and right caudate nucleus, whole hippocampus and cerebellum. Signiﬁcant increase in NTPDase and ecto 50-nucleotidase activities was observed in the injured cortex following CSI, whereas in other brain areas only an increase in ecto 50-nucleotidase activity was seen. Immunohistochemical analysis performed using antibodies speciﬁc to NTPDase 1 and ecto 50-nucleotidase demonstrated that CSI induced sig-niﬁcant changes in enzyme expression around the injury site. Immunoreactivity patterns obtained for NTPDase 1 and ecto 50-nucleotidase were compared with those obtained for glial ﬁbrillary acidic protein, as a marker of astrocytes and complement receptor type 3 (OX42), as a marker of microglia. Results suggest that up-regulation of ectonucleotidase after CSI is catalyzed by cells that activate in response to injury, i.e. cells immunopositive for NTPDase 1 were predominantly microglial cells, whereas cells immunopositive for ecto 50-nucleotidase were predominantly astrocytes.
Keywords:ATP; Adenosine; Ectonucleotidase; Ecto NTPDase; Ecto 50-nucleotidase; Cortical stab injury; Rat brain
Source:Cell Biology International, 2006, 30, 6, 541-546
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