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dc.creatorLazić, Divna
dc.creatorTešić, Vesna
dc.creatorJovanović, Mirna
dc.creatorBrkić, Marjana
dc.creatorMilanović, Desanka
dc.creatorZloković, Berislav V.
dc.creatorKanazir, Selma
dc.creatorPerović, Milka
dc.date.accessioned2020-02-14T12:43:38Z
dc.date.available2900-01-01
dc.date.issued2020
dc.identifier.issn0969-9961
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/pubmed/31931140
dc.identifier.urihttps://ibiss-r.rcub.bg.ac.rs/handle/123456789/3596
dc.description.abstractFood restriction has been widely associated with beneficial effects on brain aging and age-related neurodegenerative diseases such as Alzheimer's disease. However, previous studies on the effects of food restriction on aging- or pathology-related cognitive decline are controversial, emphasizing the importance of the type, onset and duration of food restriction. In the present study, we assessed the effects of preventive every-other-day (EOD) feeding regimen on neurodegenerative phenotype in 5XFAD transgenic mice, a commonly used mouse model of Alzheimer's disease. EOD feeding regimen was introduced to transgenic female mice at the age of 2 months and the effects on amyloid-β (Aβ) accumulation, gliosis, synaptic plasticity, and blood-brain barrier breakdown were analyzed in cortical tissue of 6-month-old animals. Surprisingly, significant increase of inflammation in the cortex of 5XFAD fed EOD mice was observed, reflected by the expression of microglial and astrocytic markers. This increase in reactivity and/or proliferation of glial cells was accompanied by an increase in proinflammatory cytokine TNF-α, p38 MAPK and EAAT2, and a decrease in GAD67. NMDA receptor subunit 2B, related to glutamate excitotoxicity, was increased in the cortex of 5XFAD-EOD mice indicating additional alterations in glutamatergic signaling. Furthermore, 4 months of EOD feeding regimen had led to synaptic plasticity proteins reduction and neuronal injury in 5XFAD mice. However, EOD feeding regimen did not affect Aβ load and blood-brain barrier permeability in the cortex of 5XFAD mice. Our results demonstrate that EOD feeding regimen exacerbates Alzheimer's disease-like neurodegenerative and neuroinflammatory changes irrespective of Aβ pathology in 5XFAD mice, suggesting that caution should be paid when using food restrictions in the prodromal phase of this neurodegenerative disease.
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173056/RS//
dc.relationNIH grant No R03AG046216
dc.rightsrestrictedAccess
dc.sourceNeurobiology of Disease
dc.subject5XFAD
dc.subjectAlzheimer's disease
dc.subjectEOD dietary restriction
dc.subjectInflammation
dc.subjectIntermittent fasting
dc.subjectNeuritic dystrophy
dc.subjectSynaptic plasticity
dc.titleEvery-other-day feeding exacerbates inflammation and neuronal deficits in 5XFAD mouse model of Alzheimer's disease.
dc.typearticleen
dc.rights.licenseARR
dcterms.abstractЈовановић, Мирна; Бркић, Марјана; Перовић, Милка; Лазић, Дивна; Тешић, Весна; Милановић, Десанка; Злоковић, Берислав В.; Каназир, Селма;
dc.rights.holder© 2020 Elsevier Inc.
dc.citation.volume136
dc.identifier.doi10.1016/j.nbd.2020.104745
dc.identifier.pmid31931140
dc.identifier.scopus2-s2.0-85077748663
dc.identifier.wos000516662200016
dc.citation.apaLazic, D., Tesic, V., Jovanovic, M., Brkic, M., Milanovic, D., Zlokovic, B. V., et al. (2020). Every-other-day feeding exacerbates inflammation and neuronal deficits in 5XFAD mouse model of Alzheimer’s disease. Neurobiology of Disease, 136, 104745.
dc.citation.vancouverLazic D, Tesic V, Jovanovic M, Brkic M, Milanovic D, Zlokovic B V., Kanazir S, Perovic M. Every-other-day feeding exacerbates inflammation and neuronal deficits in 5XFAD mouse model of Alzheimer’s disease. Neurobiol Dis. 2020;136:104745.
dc.citation.spage104745
dc.type.versionpublishedVersion
dc.citation.rankM21


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