Razvoj model sistema za ex vitro transformaciju Impatiens walleriana
Development of the model system for ex vitro transformation of Impatiens Walleriana
Cingel, Aleksandar D.
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We investigated possibility of ex vitro genetic transformation of Impatiens walleriana using Agrobacterium rhizogenes A4M70GUS and Agrobacterium tumefaciens C58C1pac1 bacterial suspension mixture with aim to obtain composite plants carrying GUS and pac1 genes. Compared to the control, treated plants exhibited increase of 27% in number of roots, while a difference in shoot length and number of leaves was not statistically significant. The PCR analysis did not confirmed integration of transgenes in the plant genome. The reason for this can be inadequate sampling or low transformation efficiency.
Keywords:A. rhizogenes A4M70GUS; A. tumefaciens C58C1pac1; ex vitro; I. Walleriana
Source:Journal of Scientific Agricultural Research, 2011, 72, 4, 23-29