Genetičke transformacije familije Brassicaceae
Transformations of Brassicaceae family
Authors:Sretenović-Rajičić, Tatjana D.
Article (Published version)
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Brassicaceae are economically very important crop family. Improvement of its characteristics is in focus for the last thirty years very much. One of the methods of biotechnology used in Brassica genus is genetic transformation. In this paper we have discussed transformations of Brassica genus, comparing it with the results that we achieved in our laboratory. We have performed inoculation with Agrobacterium tumefacien, with the construct containing resistance to herbicide Basta (bar), marker genes for Kanamycin resistance (npt II) and reporter gene-glucuronidase. We have also discussed risks of transgenic crop use and seed development.
Keywords:kupusnjače; Brassicaceae; transformations; genetičko inženjerstvo; Agrobacterium; A. tumefaciens; transgeni
Source:Selekcija i semenarstvo, 2000, 7, 1-2, 111-116