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dc.creatorChiavaroli, Annalisa
dc.creatorSinan, Koaudio Ibrahime
dc.creatorZengin, Gokhan
dc.creatorMahomoodally, Mohamad Fawzi
dc.creatorBibi Sadeer, Nabeelah
dc.creatorEtienne, Ouattara Katinan
dc.creatorCziáky, Zoltán
dc.creatorJekő, József
dc.creatorGlamočlija, Jasmina
dc.creatorSoković, Marina
dc.creatorRecinella, Lucia
dc.creatorBrunetti, Luigi
dc.creatorLeone, Sheila
dc.creatorAbdallah, Hassan H.
dc.creatorAngelini, Paola
dc.creatorAngeles Flores, Giancarlo
dc.creatorVenanzoni, Roberto
dc.creatorMenghini, Luigi
dc.creatorOrlando, Giustino
dc.creatorFerrante, Claudio
dc.date.accessioned2020-07-02T10:43:28Z
dc.date.available2020-07-02T10:43:28Z
dc.date.issued2020
dc.identifier.issn2076-3921
dc.identifier.urihttps://www.mdpi.com/2076-3921/9/6/533
dc.identifier.urihttps://ibiss-r.rcub.bg.ac.rs/handle/123456789/3719
dc.description.abstractMangrove forests exemplify a multifaceted ecosystem since they do not only play a crucial ecological role but also possess medicinal properties. Methanolic, ethyl acetate and aqueous leaf and bark extracts were prepared using homogenizer-assisted extraction (HAE), infusion and maceration (with and without stirring). The different extracts were screened for phytochemical profiling and antioxidant capacities in terms of radical scavenging (DPPH, ABTS), reducing potential (CUPRAC, FRAP), total antioxidant capacity and chelating power. Additionally, R. racemosa was evaluated for its anti-diabetic (α-amylase, α-glucosidase), anti-tyrosinase and anti-cholinesterase (AChE, BChE) activities. Additionally, antimycotic and antibacterial effects were investigated against Eescherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, Listeria monocytogenes, Enterobacter cloacae, Bacillus cereus, Micrococcus luteus, Staphylococcus aureus, Aspergillus fumigatus, Aspergillus niger, Trichoderma viride, Penicillium funiculosum, Penicillium ochrochloron and Penicillium verrucosum. Finally, based on phytochemical fingerprint, in silico studies, including bioinformatics, network pharmacology and docking approaches were conducted to predict the putative targets, namely tyrosinase, lanosterol-14-α-demethylase and E. coli DNA gyrase, underlying the observed bio-pharmacological and microbiological effects. The methanolic leave and bark extracts (prepared by both HAE and maceration) abounded with phenolics, flavonoids, phenolic acids and flavonols. Results displayed that both methanolic leaf and bark extracts (prepared by HAE) exhibited the highest radical scavenging, reducing potential and total antioxidant capacity. Furthermore, our findings showed that the highest enzymatic inhibitory activity recorded was with the tyrosinase enzyme. In this context, bioinformatics analysis predicted putative interactions between tyrosinase and multiple secondary metabolites including apigenin, luteolin, vitexin, isovitexin, procyanidin B, quercetin and methoxy-trihydroxyflavone. The same compounds were also docked against lanosterol-14α-demethylase and E. Coli DNA gyrase, yielding affinities in the submicromolar–micromolar range that further support the observed anti-microbial effects exerted by the extracts. In conclusion, extracts of R. racemosa may be considered as novel sources of phytoanti-oxidants and enzyme inhibitors that can be exploited as future first-line pharmacophores.
dc.publisherMDPI AG
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200007/RS//
dc.relationItalian Ministry of University funds (FAR 2019)
dc.rightsopenAccess
dc.sourceAntioxidants
dc.subjectAnti‐microbial activity
dc.subjectEnzyme inhibition
dc.subjectHomogenizer‐assisted extraction
dc.subjectIn silico studies
dc.subjectMangrove
dc.subjectPhytoanti‐oxidants
dc.subjectPhytochemical
dc.titleIdentification of Chemical Profiles and Biological Properties of Rhizophora racemosa G. Mey. Extracts Obtained by Different Methods and Solvents
dc.typearticleen
dc.rights.licenseBY
dcterms.abstractЦзиáкy, Золтáн; Махомоодаллy, Мохамад Фаwзи; Јекő, Јóзсеф; Гламочлија, Јасмина; Соковић, Марина; Менгхини, Луиги; Цхиавароли, Aнналиса; Зенгин, Гокхан; Синан, Коаудио Ибрахиме; Биби Садеер, Набеелах; Етиенне, Оуаттара Катинан; Рецинелла, Луциа; Брунетти, Луиги; Леоне, Схеила; Aбдаллах, Хассан Х.; Aнгелини, Паола; Aнгелес Флорес, Гианцарло; Венанзони, Роберто; Орландо, Гиустино; Ферранте, Цлаудио;
dc.rights.holder© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
dc.citation.issue6
dc.citation.volume9
dc.identifier.doi10.3390/antiox9060533
dc.identifier.pmid32570898
dc.identifier.scopus2-s2.0-85086705890
dc.identifier.wos000554024600001
dc.citation.apaChiavaroli, A., Sinan, K. I., Zengin, G., Mahomoodally, M. F., Bibi Sadeer, N., Etienne, O. K., et al. (2020). Identification of Chemical Profiles and Biological Properties of Rhizophora racemosa G. Mey. Extracts Obtained by Different Methods and Solvents. Antioxidants, 9(6), 533.
dc.citation.vancouverChiavaroli A, Sinan KI, Zengin G, Mahomoodally MF, Bibi Sadeer N, Etienne OK, Cziáky Z, Jekő J, Glamočlija J, Soković M, Recinella L, Brunetti L, Leone S, Abdallah HH, Angelini P, Angeles Flores G, Venanzoni R, Menghini L, Orlando G, Ferrante C. Identification of Chemical Profiles and Biological Properties of Rhizophora racemosa G. Mey. Extracts Obtained by Different Methods and Solvents. Antioxidants. 2020;9(6):533.
dc.citation.spage533
dc.type.versionpublishedVersion
dc.identifier.fulltexthttp://ibiss-r.rcub.bg.ac.rs/bitstream/id/6308/Antioxidants_2020_9_6_533.pdf
dc.citation.rankaM21


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