Uticaj fulerenola na aktivnost antioksidativnih enzima u ozračenoj kulturi ćelija humane eritroleukemije (K562)
The influence of fullerenol on antioxidative enzyme activity in irradiated human erythroleukemic cell line (K562)
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Cell culture K562 samples were treated with fullerenol (C6o(OH)24) at a concentration of 10 nmol/mL and thereafter irradiated with X-rays (24Gy). The activity of gamma-glutamyltransfrease (γ-GT), total superoxide-dismutase (SOD) and glutathion-peroxidase (GSH-Px) was determined 1, 24 and 48 hours after irradiation. Irradiation induces an increase in the activity of all the investigated enzymes. Fullerenol in the applied dose decreased the γ-GT activity 24 and 48 h after irradiation. The total SOD activity is increased in both pretreated groups except in the irradiated group at the 48th hour. Treatment with fullerenol before irradiation increased GSH-Px activity in irradiated groups and decreased it in the control groups.
Keywords:X-irradiation; X-zračenje; ćelijska kultura; cell culture; Fulerenol; antioxidative enzymes; antioksidativni enzimi
Source:Hemijska industrija, 2007, 61, 3, 164-166