Anxiolytic-like effects of xanthone-rich diethylether extract of Gentiana kochiana in rodents
Krstić Milošević, Dijana
Article (Published version)
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A diethylether extract of Gentiana kochiana aerial parts (GE), rich in two xanthones (gentiacaulein and gentiakochianin, 90% w/w), has already been shown to inhibit microsomal MAO enzymes in vitro and to exhibit certain antidepressant effects on mice. The aim of this study is to evaluate anxiolytic-like activity of GE in rodents by three behavioral tests, and to explore in vitro interactions of GE/xanthones with some anxiety-related elements of neurotransmission. The elevated plus maze test in rats and the staircase test in mice reveal specific anxiolytic-like influences of GE (5-20(40) mg/kg; ip), with the dose-effect ratios mainly converging to the inverted U-shaped curve. These effects are only partially analogous to the action of anxiolytic diazepam. In vitro assays demonstrate that neither GE nor the xanthones express substantial interactions with alpha(1)-adrenergic receptors, GABA(A) and GABA(B) receptors, and acetylcholine esterase. These results suggest a specific anxiolytic-like potential of GE in rodents and reduce the probability of particular neurochemical mechanisms to underlie this action.
Keywords:Gentiana kochiana; Xanthones; Anxiolytic-like activity; Elevated plus maze test; Staircase test; Binding assays; Rodents
Source:Digest Journal of Nanomaterials and Biostructures, 2011, 6, 3, 1385-1392
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