Efekti humane normalne i FALS mutirane L144P SOD1 na nevaskularne kontrakcije glatkih mišića
The effects of human wild-type and FALS mutant L144P SOD1 on non-vascular smooth muscle contractions
Oreščanin Dušić, Zorana
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Background: Mutated copper, zinc-containing superoxide dismutase (SOD1) may self-aggregate, an event that could also be an initial cause of motor neuron malfunction leading to disease onset. The effects of human mutated SOD1 protein from the blood of familial amyotrophic lateral sclerosis (FALS) patients bearing Leu144Phe (L144F) mutation were compared to wild-type (WT) human SOD1 derived from healthy examinees, for enzymatic activity and the effects on isometric contractions of non-vascular smooth muscle. Methods: We isolated WT and L144F SOD1 enzymes from eight patients with FALS, L144F mutation in exon 5 and eight healthy controls. We then investigated SOD1 activities in the obtained samples by the adrenaline method and profiled them electrophoretically. Finally, we applied WT and L144F SOD1 on the isolated rat uterus. Results: L144F SOD1 showed lower superoxide-dismutating activity compared to WT human SOD1. We found that, in contrast to WT human SOD1, mutated L144F does not induce smooth muscle relaxation. Conclusions: Our data suggest that the lack of relaxation of muscle tonus in the presence of mutated SOD1 may have pathogenic feedback effects in FALS.
Keywords:FALS; FALS; L144F SOD1; L144F SOD1; WT SOD1; WT SOD1; relaksacija; relaxation
Source:Journal of Medical Biochemistry, 2013, 32, 4, 375-379