In vitro umnožavanje u kulturi segmenata stabla of Dianthus ciliatus ssp. dalmaticus i D. giganteus ssp. croaticus (Caryophyllaceae)
In vitro propagation of Dianthus ciliatus ssp. dalmaticus and D. giganteus ssp. croaticus (Caryophyllaceae) from stem segment cultures
Ćalić-Dragosavac, Dušica D.
Article (Published version)
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Plant regeneration of carnation species Dianthus ciliatus ssp. dalmaticus and Dianthus giganteus ssp. croaticus was achieved through micropropagation from apical and nodal segments culture on MS2. Shoots multiplication was successful on the same medium via axillary buds. There were differences between multiplication index (MI) of shoots originating from apical and nodal basal stem segments. Nodal segment shoots had a better MI (D. ciliatus ssp. dalmaticus = 7.86; D. giganteus ssp. croaticus = 0.68) than apical ones (D. ciliatus ssp. dalmaticus = 6.94; D. giganteus ssp. croaticus = 0.50). Shoots of both species were rooted on MS0 without hormones, MS3 and MS4. Adventitious buds (AB) and somatic embryo like structures (ES) were formed after the transfer of green-yellow callus from MS5 to MS6. Further development and multiplication of AB and ES were achieved on medium MS7. Plants formation was brought about by micropropagation of shoots, organogenesis and/or somatic embryogenesis. In vitro plantlets of both carnation species were planted in rocky garden of the Belgrade Botanical Garden 'Jevremovac' where they bloomed. Subsequently, these in vitro plantlets will be reintroduced in natural environment. .
Keywords:Dianthus ciliatus ssp. dalmaticus; Dianthus ciliatus ssp. dalmaticus; Dianthus giganteus ssp. croaticus; Dianthus giganteus ssp. croaticus; adventivni pupoljci; adventitious buds; strukture slične embrionu; somatic embriogenesis; somatic embryo like structure; organogeneza; kultura segmenata stabla; micropropagation; organogenesis; stem segment culture
Source:Botanica Serbica, 2010, 34, 2, 153-161